Stopped-flow time difference analysis. A review.
نویسندگان
چکیده
منابع مشابه
Determination of the dead time of a stopped-flow fluorometer.
This investigation was carried out to develop a convenient alternative method for examining the performance and determining the dead time of a stopped-flow fluorometer. We examined the kinetics for the formation of the fluorescent Mg2+-8-hydroxyquinoline chelate in aqueous solutions. The reversible association of the Mg2+ ion with 8-hydroxyquinoline is a second-order process whose on and off ra...
متن کاملStopped-flow kinetic analysis using Hadamard transform time-of-flight mass spectrometry.
A home-built stopped-flow apparatus is interfaced to a Hadamard transform time-of-flight mass spectrometer, which permits study of reaction kinetics with a time between reaction initiation and observation as short as about 100 ms and a sampling rate of chemical change that can approach 1 ms. This technique is applied to the trypsin-catalyzed hydrolysis of several peptides and is validated by co...
متن کاملStopped-flow kinetic analysis of the bacterial luciferase reaction.
The kinetics of the reaction catalyzed by bacterial luciferase have been measured by stopped-flow spectrophotometry at pH 7 and 25 degrees C. Luciferase catalyzes the formation of visible light, FMN, and a carboxylic acid from FMNH2, O2, and the corresponding aldehyde. The time courses for the formation and decay of the various intermediates have been followed by monitoring the absorbance chang...
متن کاملStopped Flow Spectrophotometric Observation of Superoxide
There are few ways to develop a high concentration of superoxide in solutions of physiological significance, and by far the most useful of these, pulsed radiolysis of oxygenated solutions [1 ], requires very specialized and expensive research equipment. One of the long term goals of this laboratory has been to develop alternative procedures for examining the chemistry of superoxide ion in aqueo...
متن کاملa time-series analysis of the demand for life insurance in iran
با توجه به تجزیه و تحلیل داده ها ما دریافتیم که سطح درامد و تعداد نمایندگیها باتقاضای بیمه عمر رابطه مستقیم دارند و نرخ بهره و بار تکفل با تقاضای بیمه عمر رابطه عکس دارند
ذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analytical Sciences
سال: 1988
ISSN: 0910-6340,1348-2246
DOI: 10.2116/analsci.4.445